Some evidence for the occurrence of ribonucleic acid in the lutoid fraction (lysosomal compartment) from Hevea brasiliensis latex.
نویسندگان
چکیده
Some results are reported for the presence of some RNA species in the lutoid fraction from Hevea brasiliensis latex. The origin of this RNA is discussed.
منابع مشابه
The isolation and composition of helical protein microfibrils from Hevea brasiliensis latex.
1. The microfibrils contained within the lutoid particles of Hevea brasiliensis latex obtained from young tissue have been isolated by methods based on low-speed centrifugation, isoelectric precipitation and gel filtration. 2. The isolated microfibrils behave as a single protein having an isoelectric point of about 4 as determined by paper electrophoresis. 3. The only components so far detected...
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Lutoids represent a lysosomal microvacuolar compartment of rubber-tree (Hevea brasiliensis) latex. We observed acidification of isolated vesicles after imposing an outward Mg(2+) diffusion gradient and dissipation of a preformed pH gradient in the presence of exogenous Mg(2+). These results suggest the presence of a Mg(2+)/H(+) antiporter. The maximum Mg(2+)/H(+) exchange rate was observed at p...
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An in vitro aggregation of washed lutoid membrane and rubber particles, respectively, prepared from the bottom (lutoid) fraction and rubber layer of centrifuged fresh latex, leading to the formation of rubber coagulum necessary for a latex coagulation was demonstrated. A Triton X-100 extract of washed lutoid membrane proteins, isolated and prepared from the bottom fraction of centrifuged fresh ...
متن کاملMolecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis.
Natural rubber from Hevea brasiliensis is a high molecular mass polymer of isoprene units with cis-configuration. The enzyme responsible for the cis-1,4-polymerization of isoprene units has been idengified as a particle-bound rubber transferase, but no gene encoding this enzyme has been cloned from rubber-producing plants. By using sequence information from the conserved regions of cis-prenyl c...
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عنوان ژورنال:
- The Biochemical journal
دوره 143 2 شماره
صفحات -
تاریخ انتشار 1974